Liens utiles

Au cours des dernières années, on a assisté à une explosion des sites Web éducatifs consacrés à la microscopie. Certains des sites les plus utiles sont énumérés ci-dessous :

Ressources toujours à jour (jobs, évènements, éducation) sur Focal Plane

Sample preparations and dyes- A great sample preparation resource for techniques, applications and dyes for many needs. 

Molecular Expressions Optical Microscopy Primer- a great microscopy resource which discusses many aspects of microscopy in an understandable fashion. Their virtual microscopes are informative as well as fun.

Zeiss Campus- The Carl Zeiss Microscopy Online Campus website explores the fascinating world of optical microscopy and provides the necessary background to understand both the basic concepts and advanced principles.

Olympus Microscopy Resource Center- includes a microscopy primer as well as information about general microscopy, confocal microscopy, digital imaging, and advanced techniques.

Nikon MicroscopyU - Another useful resource for information on confocal as well as general microscopy.

MyScope-training for advanced research - Useful resource for information on confocal as well as general microscopy, with a final quiz to test your microscopy knowledge

Quantifying Microscopy Images: 10 Tips for Image acquisition - a Quick summary of what you should do and don't. Because with the "power of Microscopy comes Responsability and no amount of fancy image analysis can overcome image acquisition problems" Prof. Anne Carpenter

• Colour me better: fixing figures for colour blindness
• Publishers unite to tackle doctored images in research papers and Check the guide about Image integrity
• Creating Clear and Informative Image-based Figures for Scientific Publications
• Effective image vizualization for publications
• Tutorial: guidance for quantitative confocal microscopy
• Sharper signals: how machine learning is cleaning up microscopy images
• The hunt for red fluorescet proteins
• Reproductibilité
• Reproductibilité et Métadonnées

• Numerical Aperture and resolution

Vous trouverez ci-dessous quelques sites utiles contenant des bases de données de spectres d'excitation/émission de fluorophores. Il s'agit d'excellentes ressources pour évaluer la séparation spectrale et le débordement de vos fluorophores avant de réaliser vos expériences. Vous pouvez également utiliser ces ressources pour visualiser les spectres et comparer les options de vos fluorophores aux jeux de filtres disponibles sur les microscopes de l'installation:

• FPbase
• Spectraviewer
• Chroma Spectra Viewer

• Antibody database basé sur des articles scientifiques déjà publiés.
• Article dans Nature : "When antibodies mislead: the quest for validation". Read the article

• Fiji- a useful image processing program derived from ImageJ, with many plugins already included.
• CellProfiler and CellProfiler Analyst- open source HCS image analysis software available from the Broad Institute.