Instruments

Bioscience 411
Liquids 1H/13C/31P/19F/2H QNP probe with Z gradient

Bioscience 411
Liquids autotuning broadband probe (BBFO) with Z gradient

Summary

Location: Biosciences Complex room 411.
Installation Date: June 2^nd, 2025 (Console and iProbe).
Funding Source: CFI-JELF, 2023.

The Bruker Avance Neo spectrometer operates using a 9.4 T (400 MHz) Ultrashield standard bore magnet (originally installed on October 20^th, 2005). The NMR spectrometer is normally equipped with a Bruker triple resonance broadband observe (TBO) iProbe capable of observing almost any nucleus from ¹⁰⁹Ag to ³¹P. The outer coil is tuned for ¹H decoupling or observation, and a dedicated ¹⁹F channel allows for direct fluorine observation or decoupling. It features a fully automated 60-slot robotic sample changer, allowing users to walk up and schedule their samples for analysis.

Equipped with the TBO iProbe, this instrument is ideal for routine spectroscopy of X, F and H nuclei, and offers exceptional sensitivity. Simultaneous ¹H and ¹⁹F decoupling offers a unique advantage for fluorinated molecules (organofluorine small molecules, pharmaceuticals, materials, polymers, etc.).

Nucleus	90° pulse duration (μs)	90° pulse power (W)	Power Limit (W)
¹H	10.0	17.3	40
¹³C	9.9	54.9	140
¹⁵N	20.9	65.1	207
¹⁹F (BB)	12.0	33.9	90
¹⁹F	24.1	34.4	72
²H	639.3	1.1	50
³¹P	10	34.5	70

Nucleus	Signal-to-noise	Remarks
¹H	523.1
¹³C	259.2
¹³C	323.3	With 1H Decoupling
¹⁵N	41.9	With 1H Decoupling
¹⁹F (BB)	321.7
¹⁹F (BB)	634.9	Using broadband coil
¹⁹F	178.9
²H	1500.6	10% D₂O
³¹P	261.8

Nucleus	50 % (Hz)	0.55 % (Hz)	0.11 % (Hz)	Remarks
¹H	0.53	4.4	8.3	Without rotation
¹H	0.34	3.0	5.8	With rotation
¹³C	0.06	0.9	2.4	With rotation

Summary

Location: D'Iorio Hall room 416.

The Bruker Avance III HD spectrometer operates using a 14.1 T (600 MHz) Ultrashield standard bore magnet. The NMR spectrometer is normally equipped with a Bruker triple resonance inverse (TCI) cryoprobe optimized for ¹H and ¹³C observation. It features a fully automated robotic sample changer, allowing users to walk up and schedule their samples for analysis.

Equipped with the cryoprobe, this instrument is ideal for high-sensitivity biological NMR research, and applications requiring high sensitivity and resolution.

Spectrometer Features:

SampleCase 24-slot robotic sample changer for automated NMR acquisition (including automatic tuning, lock and shim).
Autocalibrate: The instrument is automatically calibrated daily at 5:00 am, ensuring high quality data.
BCU I chiller for variable temperature experiments (-30 °C to +150 °C).
Topspin 3.6.5 with IconNMR for automation.

Probes

TCI: Triple-Resonance Inverse Cryoprobe (normally installed for operation)

Ideal for protein NMR and standard small-molecule experiments requiring high sensitivity.
Experiments: ¹H, ¹³C, ¹⁵N, HSQC, HMQC, HMBC, COSY, NOESY, Water Suppression techniques
Tuning Configuration: H-C/N-D
Temperature range: -40 °C to +80 °C
Autotuning: yes
Gradient: yes