Zeiss AxioObserver D1

The CBIA Core is home to a variety of wide field epifluorescent microscopes to suit routine imaging needs in both live and fixed samples.

Microscope specifications

Zeiss AxioObserver D1 microscope
Stand modelAxioObserver D1 [inverted]
Microscope controls
  • Fully automated reflector turret
  • Manual objective nose piece, stage control (x, y), focus control (z), light path control and  condenser
Software Zen Blue 2.3
Capabilities
  • Epifluorescence imaging
  • Brightfield, phase contrast and DIC
  • Multi-channel imaging
  • Timelapse
Objectives
  • 10x, 0.25 NA, Air, N-AchroPlan (Ph1)
  • 20x, 0.40 NA, Air, LD Plan NeoFluar Corr. (Ph2)
  • 20x, 0.80 NA, Air, Plan Apochromat (DIC II)
  • 40x, 0.60 NA, Air, LD Plan NeoFluar Corr. (DIC II)
  • 40x, 1.30 NA, Oil, Plan NeoFLura (Ph3)
  • 63x, 1.40 NA, Oil, Plan Apochromat (DICIII)
Light sources
  • Colibri LED (365nm, 470nm, 505nm, 555nm)
  • Transmitted LED source
Filters
  • Blue Ex 390/22nm, FT 420nm, Em 460/50nm
  • Green Ex 470/40nm, FT 495nm, Em 525/50nm
  • Yellow Ex 500/25nm, FT 515nm, Em 535/30nm
  • Red Ex 560/40, FT 585nm, Em 630/75nm
  • Far Red Ex 640/30, FT 660, Em 690/50nm
DetectorsAxioCam MRm CCD (monochrome)
LocationRGN 3227