Zeiss AxioObserver 7- SoFia

The CBIA Core is home to a variety of wide field epifluorescent microscopes to suit routine imaging needs in both live and fixed samples.

Microscope specifications

Observer7_SoFia
Stand modelZeiss AxioObserver 7 SoFia[Inverted]
Microscope controls
  • Fully automated objective nosepiece, reflector turret and condenser
  • Fully motorized stage control (x, y), focus control (z) and light path control
SoftwareZen 3.10 Pro
Capabilities
  • Epifluorescence imaging
  • Brightfield, phase contrast
  • Multi-channel imaging (from 405nm (DAPI) to 850 nm (Cy7 or Alexa Fluor 750))
  • Timelapse
  • Z-stack
  • Tiling and Multi-position (Unique: 4 slides holder)
  • Sample Finder
  • Definite Focus3
  • BioLabs App
Objectives
Light sources

Transmitted Light LED

Colibri 7, LED lines: 385nm, 430nm, 475nm, 555nm, 590nm, 630nm, 735nm

Emission Filters

Pentafilter Filter Set 112 HE LED: PBP 425/30 + 514/31 + 592/25 + 681/45 + 785/38

Quadfilter Filter Set 110 HE LED: QBP 425/30 + 524/51 + 634/38 + 785/38

Single filter Filter Set 112 LED SBP: 

BP 425/30
BP 592/24
BP 681/45
BP 788/38
BP 480/40

CameraZeiss Axiocam 705 monochrome camera
LocationRGN 3144