Services

The Biological John L. Holmes Mass Spectrometry Facility at the University of Ottawa provides support for high quality research activities and enhances the educational experience of students.

Proteomics

  • Protein identification in complex mixtures (biomarker discovery)
  • Post-translational modification analysis (phosphorylation, N-acetylation, glycosylation etc.)
  • Quantitative proteomics
    • MS-based on stable isotope labeling method
    • Label-free (MS1 peak integration/intensity, counting MS2 spectra)
  • Identification of interacting proteins (protein complexes analysis, protein-protein interactions)
  • DNA-, RNA- protein interactions
  • Targeted proteomics (PRMs)
  • Multiple stage fragmentation capabilities: HCD, CID and ETD
  • Proteomic based on MS3 level [tandem mass tag (TMT), Isobaric tags for relative and absolute quantitation (iTRAQ), phosphoproteomics, cross-linked peptides]

Metabolomics

  • Mass Determination
  • Natural abundance of isotopes
  • Structural characterization/elucidation (full scan and fragmentation pattern)
  • Quantification
  • Targeted and untargeted metabolomics
  • Protein-ligand interaction

Bioinformatics

  • Protein and peptide analysis using bottom-up proteomics
    1. Identification and relative label-free quantification of proteins and peptides (MaxQuant and Proteome Discoverer)
    2. Identification of various post-translational and chemical protein modifications (phosphoproteins, protein acetylation, biotinylation and others)

  • Statistical analysis of proteomics data

    1. One-group (correlation analysis), two-group (t-test), and multi-group (ANOVA) hypothesis testing
    2. Supervised and unsupervised dimension reduction (PCA, PLS-DA)
    3. Hierarchical clustering

Contact Us

Biological facility

Zoran Minic
zminic@uottawa.ca
Marion Hall - Room 02
Ottawa, ON K1N 1A2
Tel: 613-562-5800 ext. 1626